Glycomics & Sulfoglycomics
From mass spectrometry-based glycosylation analysis to glycomics and glycoproteomics. (2014). Khoo KH. Adv Neurobiol 9: 129-164.
Increasing the depth of mass spectrometry-based glycomic coverage by additional dimensions of sulfoglycomics and target analysis of permethylated glycans. (2013). Cheng PF, Snovida S, Ho MY, Cheng CW, Wu AM and Khoo KH. Anal Bioanal Chem 405(21): 6683-95.
Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue. (2013). Patnode ML, Cheng CW, Chou CC, Singer MS, Elin MS, Uchimura K, Crocker PR, Khoo KH and Rosen SD. J Biol Chem 288(37): 26533-45.
KSGal6ST generates galactose-6-O-sulfate in high endothelial venules but does not contribute to L-selectin-dependent lymphocyte homing. (2013). Patnode ML, Yu SY, Cheng CW, Ho MY, Tegesjo L, Sakuma K, Uchimura K, Khoo KH, Kannagi R and Rosen SD. Glycobiology 23(3): 381-94.
Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins. (2013). Wang SH, Tsai CM, Lin KI and Khoo KH. Glycobiology 23(6): 677-89.
Priming mass spectrometry-based sulfoglycomic mapping for identification of terminal sulfated lacdiNAc glycotope. (2013). Yu SY, Chang LY, Cheng CW, Chou CC, Fukuda MN and Khoo KH. Glycoconj J 30(2): 183-94.
Human sperm binding is mediated by the sialyl-Lewis(x) oligosaccharide on the zona pellucida. (2011). Pang PC, Chiu PC, Lee CL, Chang LY, Panico M, Morris HR, Haslam SM, Khoo KH, Clark GF, Yeung WS and Dell A. Science 333(6050): 1761-4.
MS-based glycomic strategies for probing the structural details of polylactosaminoglycan chain on N-glycans and glycoproteomic identification of its protein carriers. (2011). Wang SH, Wu SW and Khoo KH. Proteomics 11(14): 2812-29.
Terminal disialylated multiantennary complex-type N-glycans carried on acutobin define the glycosylation characteristics of the Deinagkistrodon acutus venom. (2011). Lin CW, Chen JM, Wang YM, Wu SW, Tsai IH and Khoo KH. Glycobiology 21(4): 530-42.
Mass spectrometric analysis of sulfated N- and O-glycans. (2010). Khoo KH and Yu SY. Methods Enzymol 478: 3-26.
Enabling techniques and strategic workflow for sulfoglycomics based on mass spectrometry mapping and sequencing of permethylated sulfated glycans. (2009). Yu SY, Wu SW, Hsiao HH and Khoo KH. Glycobiology 19(10): 1136-49.
Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment. (2007). Mitoma J, Bao X, Petryanik B, Schaerli P, Gauguet JM, Yu SY, Kawashima H, Saito H, Ohtsubo K, Marth JD, Khoo KH, von Andrian UH, Lowe JB and Fukuda M. Nat Immunol 8(4): 409-18.
Protein Glycosylation and Glycproteomics
Novel LC-MS(2) Product Dependent Parallel Data Acquisition Function and Data Analysis Workflow for Sequencing and Identification of Intact Glycopeptides. (2014). Wu SW, Pu TH, Viner R and Khoo KH. Anal Chem 86(11): 5478-5486
An Adaptive Workflow Coupled with Random Forest Algorithm to Identify Intact N-glycopeptides Detected from Mass Spectrometry. (2014). Liang SY, Wu SW, Pu TH, Chang FY and Khoo KH. Bioinformatics 30(13): 1908-1916
Sweet-Heart - an integrated suite of enabling computational tools for automated MS2/MS3 sequencing and identification of glycopeptides. (2013). Wu SW, Liang SY, Pu TH, Chang FY and Khoo KH. J Proteomics 84: 1-16.
Rapid glycopeptide enrichment and N-glycosylation site mapping strategies based on amine-functionalized magnetic nanoparticles. (2012). Kuo CW, Wu IL, Hsiao HH and Khoo KH. Anal Bioanal Chem 402(9): 2765-76.
PTP-Cys Modifications & Proteomics
Characterization of Protein Serotonylation via Bioorthogonal Labeling and Enrichment. (2014). Lin JC, Chou CC, Tu Z, Yeh LF, Wu SC, Khoo KH and Lin CH. J Proteome Res 13(8): 3523-3529
Mass Spectrometry-Based Quantitative Proteomics for Dissecting Multiplexed Redox Cysteine Modifications in Nitric Oxide-Protected Cardiomyocyte Under Hypoxia. (2014). Pan KT, Chen YY, Pu TH, Chao YS, Yang CY, Bomgarden RD, Rogers JC, Meng TC and Khoo KH. Antioxid Redox Signal 20(9): 1365-1381.
Evaluation of Drosophila metabolic labeling strategies for in vivo quantitative proteomic analyses with applications to early pupa formation and amino acid starvation. (2013). Chang YC, Tang HW, Liang SY, Pu TH, Meng TC, Khoo KH and Chen GC. J Proteome Res 12(5): 2138-50.
In vivo tagging and characterization of S-glutathionylated proteins by a chemoenzymatic method. (2012). Chiang BY, Chou CC, Hsieh FT, Gao S, Lin JC, Lin SH, Chen TC, Khoo KH and Lin CH. Angew Chem Int Ed Engl 51(24): 5871-5.
Nitrite-mediated S-nitrosylation of caspase-3 prevents hypoxia-induced endothelial barrier dysfunction. (2011). Lai YC, Pan KT, Chang GF, Hsu CH, Khoo KH, Hung CH, Jiang YJ, Ho FM and Meng TC. Circ Res 109(12): 1375-86.
Cysteine S-nitrosylation protects protein-tyrosine phosphatase 1B against oxidation-induced permanent inactivation. (2008). Chen YY, Chu HM, Pan KT, Teng CH, Wang DL, Wang AH, Khoo KH and Meng TC. J Biol Chem 283(50): 35265-72.